Cost & Usage
pE300 White: LED White Illumination System
Principal Use: Simple-to-use LED illumination system
CoolLED’s pE-300-White is designed to offer broad-spectrum LED illumination for general use in fluorescence applications. The unit can be fitted directly to the microscope as a better and safer alternative to a high-pressure mercury or metal-halide light source. Spectral coverage is from the UV (DAPI excitation) to the Red region (Cy5 excitation). The unit will excite common fluorophores used in hospital and research laboratory environments.
With a comprehensive range of microscope adaptors, the pE-300-White can be fitted to most current and older microscopes. The result is a safe, convenient light-source which will last for many years without any additional operating costs.
Advantages of the pE-300-White
- Broad spectrum, high power white illumination
- Simple to fit, simple to use
- Instant On/Off
- Excellent Field of View
- No bulb replacement or aligning
- Long lifetime (25,000 operating hours)
- Reduced power of consumption for energy saving
- No shutters or ND filters required
- Filter set compatibility, including DAPI
- Stable and repeatable
- Quiet operation
- Fits all new and most older microscopes
- TTL triggering
- No mercury
Simply Better Control
The manual Control Pod offers instant on/off operation for White illumination with 0-100% intensity control in 1% steps.
The pE-300-White is ideal for use with multi-band filter sets providing simplicity of operation with fewer filter cubes – potentially removing the need for single band filter sets altogether. It allows you to control the brightness of each fluorophore independently, in a multi-stained sample. Fluorophores can be viewed in isolation or in combination without filter cube changes.
Application example: FISH
Liquid Light-Guide Option
The pE-300 can be supplied with a liquid light-guide when there is a need to keep the source of illumination remote from the microscope. An optional collimating adaptor is available if required. As a replacement to a metal-halide source on a confocal microscope, it can be used for pre-aligning samples before scanning commences. This is particularly attractive as photo-bleaching of the sample can be minimised when LED illumination is used.